…Furthermore, in 1929, Fell perfected the watch glass culture method together with Robinson R. Robinson. In 1952, E. Wolff and K. Haffen of France developed an organ culture method using nutrients and a semi-solid substrate suitable for organ growth, which was used in research on sex differentiation, differentiation of embryonic organs, cancer, and other subjects. Meanwhile, with the development of a cell dissociation method using proteolytic enzymes such as trypsin (Moscona A. Moscona, 1952), cell culture methods were established, and cell lines, cell strains, and cell clones capable of subculturing mammalian cells were established one after another using this method. … *Some of the terminology that mentions "Wolf, É." is listed below. Source | Heibonsha World Encyclopedia 2nd Edition | Information |
…さらに,フェルは29年に,ロビンソンR.Robinsonとともに時計皿培養法を完成した。52年になると,フランスのウォルフÉ.WolffとアッフェンK.Haffenとは,器官的成長に適した栄養分と半固体の基質からなる器官培養法を開発して,性分化現象,胚器官の分化,癌などの研究に供した。一方では,トリプシンなどのタンパク質分解酵素による細胞解離法の開発(モスコーナA.Moscona,1952)に伴って細胞培養法が確立し,これを利用した哺乳類細胞による継代培養が可能な細胞系cell line,細胞株cell strain,細胞クローンが次々と樹立された。… ※「ウォルフ,É.」について言及している用語解説の一部を掲載しています。 出典|株式会社平凡社世界大百科事典 第2版について | 情報 |
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