An extremely sensitive method that uses antibodies or antigens labeled with fluorescent dyes such as fluorescein or rhodamine to detect antigens or antibodies present in body fluids, tissues, etc. Of these, when antibodies are labeled, it is often called the fluorescent antibody method. There are two methods: direct and indirect. In the case of fluorescent antibodies, the direct method involves reacting a fluorescently labeled antibody that specifically binds to an antigen present in the tissue with a thin tissue slice, and when the slice is thoroughly washed and observed under a fluorescent microscope, the fluorescent antibody that has bound to the antigen in the tissue emits fluorescence, revealing the presence of that antigen. Source: Heibonsha World Encyclopedia, 2nd Edition Information |
抗体または抗原にフルオレセインやローダミンのような蛍光色素を標識したものを用いて,体液,組織などに存在する抗原または抗体を検出する,きわめて感度の高い方法。このうち,抗体を標識する場合は蛍光抗体法と呼ぶことが多い。直接法と間接法の2法がある。蛍光抗体の場合についていえば,直接法は組織中に存在する抗原に対して特異的に結合する蛍光標識抗体を,薄い組織切片に反応させ,この切片をよく洗って,蛍光顕微鏡で観察すると,組織中の抗原と結合した蛍光抗体が蛍光を発するので,その抗原の存在を知ることができる。
出典 株式会社平凡社世界大百科事典 第2版について 情報 |
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