Reverse dilution method

Japanese: 逆希釈法 - ぎゃくきしゃくほう
Reverse dilution method

…It is extremely difficult to quantitatively separate the target substance from the sample to be analyzed in a pure form, but this method requires only a pure separation regardless of the yield, and can provide reliable results with relatively simple operations. When the target substance is non-radioactive and radioactive, these methods are called the direct dilution method and the inverse dilution method, respectively. (1) Direct dilution method When the amount x of the target compound in a sample is unknown, add W of the same compound labeled with a radioisotope whose specific activity S 1 is known, mix well, and then separate the compound in a pure form and measure the specific activity S 2 to determine x as follows. …

*Some of the terminology that refers to the "inverse dilution method" is listed below.

Source | Heibonsha World Encyclopedia 2nd Edition | Information

Japanese:

…分析対象となる試料から目的とする物質を定量的に純粋に分離することはきわめて困難であるが,この方法によれば,収率に関係なく,純粋に分離を行いさえすればよく,比較的簡単な操作で信頼性の高い結果が得られる。 分析対象となる物質が非放射性および放射性の場合,おのおの直接希釈法および逆希釈法と呼ばれる。(1)直接希釈法 試料中の目的とする化合物の量xが未知であるとき,比放射能S1のわかった放射性同位元素で標識した同じ化合物をWだけ加え,よく混合した後その化合物を純粋に分離して,比放射能S2を測定すれば,次のようにxを求めることができる。…

※「逆希釈法」について言及している用語解説の一部を掲載しています。

出典|株式会社平凡社世界大百科事典 第2版について | 情報

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