… In actual research, the question is how to isolate the gene of interest. Isolating a gene is called gene cloning. There are two typical methods: one is to prepare complementary DNA (cDNA) from messenger RNA using reverse transcriptase (RNA-dependent DNA polymerase), and the other is the transformation method. … *Some terminology explanations that mention "cloning" are listed below. Source | Heibonsha World Encyclopedia 2nd Edition | Information |
… 実際に研究を行うにあたっては,着目する遺伝子をいかにして単離するかが問題となる。遺伝子の単離を遺伝子のクローニングcloningという。代表的方法は二つあり,一つはメッセンジャーRNAから逆転写酵素(RNA依存性DNAポリメラーゼ)によって相補的DNA(cDNA)を調製するもので,もう一つは形質転換法である。… ※「cloning」について言及している用語解説の一部を掲載しています。 出典|株式会社平凡社世界大百科事典 第2版について | 情報 |
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