Nuclear transfer method

...After serving as a lecturer at the same university, he is now a member of the British Medical Research Foundation's Institute of Molecular Biology. Using African clawed frogs and leopard frogs, he found that when unfertilized eggs were exposed to ultraviolet light to inactivate the nuclei, and the nuclei of differentiated small intestinal epithelial cells were then inserted into them (nuclear transfer method), normal tadpoles were generated. He also found that tadpoles were obtained when the nuclei of early frog embryos generated by nuclear transfer were inserted into unfertilized eggs that had been treated with ultraviolet light (passive nuclear transfer method). ...

*Some of the terms used in the "nuclear transfer method" are listed below.

Source | Heibonsha World Encyclopedia 2nd Edition | Information

…同大講師を経て英国医学研究振興会分子生物学研究所所員。アフリカツメガエルやヒョウガエルを用い，未受精卵に紫外線照射をして核を不活性化し，そこへ分化が進んだ小腸上皮細胞の核を挿入する(核移植法)と正常なオタマジャクシが発生することを見いだした。また，核移植をして発生させたカエル初期胚の核を紫外線処理した未受精卵に挿入(継代核移植法)してもオタマジャクシが得られた。…

※「核移植法」について言及している用語解説の一部を掲載しています。

出典｜株式会社平凡社世界大百科事典 第２版について | 情報