…In 1952, E. Wolff and K. Haffen of France developed an organ culture method using nutrients and a semi-solid substrate suitable for organ growth, which was used to study sex differentiation, embryonic organ differentiation, cancer, and other subjects. On the other hand, with the development of a cell dissociation method using proteolytic enzymes such as trypsin (A. Moscona, 1952), cell culture methods were established, and cell lines, cell strains, and cell clones capable of subculture using mammalian cells were established one after another using this method. In 1958, F. C. Steward succeeded in regenerating a complete individual from a single cultured cell obtained from the cambium of a carrot. … *Some of the terminology that refers to "cell line" is listed below. Source | Heibonsha World Encyclopedia 2nd Edition | Information |
…52年になると,フランスのウォルフÉ.WolffとアッフェンK.Haffenとは,器官的成長に適した栄養分と半固体の基質からなる器官培養法を開発して,性分化現象,胚器官の分化,癌などの研究に供した。一方では,トリプシンなどのタンパク質分解酵素による細胞解離法の開発(モスコーナA.Moscona,1952)に伴って細胞培養法が確立し,これを利用した哺乳類細胞による継代培養が可能な細胞系cell line,細胞株cell strain,細胞クローンが次々と樹立された。また58年にはF.C.スチュワードがニンジンの形成層から得た1個の培養細胞から完全な個体を再生することに成功した。… ※「cell line」について言及している用語解説の一部を掲載しています。 出典|株式会社平凡社世界大百科事典 第2版について | 情報 |
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