Ouchterlony's method

Japanese: Ouchterlony's method(英語表記)Ouchterlonysmethod
Ouchterlony's method

…The principle behind the formation of precipitation lines is the same as in general precipitation reactions, where the precipitate forms at the location where the concentration ratio of the antigen and antibody substances diffusing within the gel is optimal. The Ouchterlony's method is widely used. In this method, multiple holes are made in an agar plate, and when an antigen solution and serum containing antibodies (antisera) are diffused face-to-face, precipitation lines form between the holes, and precipitation lines can be observed according to the number of antigen-antibody reaction systems. …

*Some of the terminology that refers to "Ouchterlony's method" is listed below.

Source | Heibonsha World Encyclopedia 2nd Edition | Information

Japanese:

…沈降線ができる原理は,一般の沈降反応と同じで,ゲル内を拡散する抗原,抗体両物質の濃度比が最適の場所で沈降物が形成されることによる。広く用いられるのはオークテルロニー法Ouchterlony’s methodである。これは,寒天平板に複数の孔をあけ,抗原液と抗体を含む血清(抗血清)とを向かい合わせて拡散させると,孔の間に沈降線が生じ,抗原抗体反応系の数に応じた沈降線が観察できる。…

※「Ouchterlony's method」について言及している用語解説の一部を掲載しています。

出典|株式会社平凡社世界大百科事典 第2版について | 情報

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